John Kucklick

National Institute of Standards and Technology

Address: Hollings Marine Laboratory, 331 Fort Johnson Road, Charleston, SC 29412
Phone: 843.460.9886
Personal Website:


Ph.D., 1992, University of South Carolina 
M.S., 1988, University of North Carolina at Wilmington

Research Interests

  • environmental and marine chemistry
  • analytical methods for biological molecules and contaminants
  • pollutant bioaccumulation and biomagnification in marine organisms
  • contaminants of emerging concern
  • marine organisms as indicators of pollution

Current and planned research projects

  • linking exposure in marine organisms to health effects
  • understanding if and why chemical contaminant concentrations are going up or down in the marine environment
  • developing non targeted mass spectrometry techniques to assess the exposome
  • bioaccumulation and new pollutants in marine organisms


  • Boggs, A., Bowden, J., Galligan, T., Guillette Jr., L., Kucklick, J. “Development of a multi-class steroid hormone screening method using liquid chromatography/tandem mass spectrometry (LC-MS/MS).” Anal Bioanal Chem 408, 4179-4190 (2016).
  • Colabuano, F., Vander Pol, S., Huncik, K., Taniguchi, S., Petry, M., Kucklick, J., Montone, R. “Persistent organic pollutants in blood samples of Southern Giant Petrels (Macronectes giganteus) from the South Shetland Islands, Antarctica,” Environ Pollut 216, 38-45 (2016).
  • Mancia, A., Abelli, L., Kucklick, J. R., Rowles, T. K., Wells, R. S., Balmer, B. C., Hohn, A. A., Baatz, J. E., and Ryan, J. C., "Microarray applications to understand the impact of exposure to environmental contaminants in wild dolphins (Tursiops truncatus)," Marine Genomics, 19, 47-57 (2015).
  • Shiffman, D. S., Frazier, B. S., Kucklick, J. R., Abel, D., Brandes, J., and Sancho, G., "Feeding Ecology of the Sandbar Shark in South Carolina Estuaries Revealed through delta C-13 and delta N-15 Stable Isotope Analysis," Marine and Coastal Fisheries, 6, 156-169 (2014).
  • Parrott, B. B., Bowden, J. A., Kohno, S., Cloy-McCoy, J. A., Hale, M. D., Bangma, J. T., Rainwater, T. R., Wilkinson, P. M., Kucklick, J. R., and Guillette, L. J., "Influence of tissue, age, and environmental quality on DNA methylation in Alligator mississippiensis," Reproduction, 147, 503-513 (2014).
  • Mancia, A., Ryan, J. C., Van Dolah, F. M., Kucklick, J. R., Rowles, T. K., Wells, R. S., Rosel, P. E., Hohn, A. A., and Schwacke, L. H., "Machine learning approaches to investigate the impact of PCBs on the transcriptome of the common bottlenose dolphin (Tursiops truncatus)," Marine Environmental Research, 100, 57-67 (2014).
  • Bowden, J. A., Bangma, J. T., and Kucklick, J. R., "Development of an Automated Multi-Injection Shotgun Lipidomics Approach Using a Triple Quadrupole Mass Spectrometer," Lipids, 49, 609-619 (2014).
  • Hoguet, J., Keller, J. M., Reiner, J. L., Kucklick, J. R., Bryan, C. E., Moors, A. J., Pugh, R. S., and Becker, P. R., "Spatial and temporal trends of persistent organic pollutants and mercury in beluga whales (Delphinapterus leucas) from Alaska," Sci. Total Environ., 449, 285-294 (2013).
  • Ellisor, D., McLellan, W., Koopman, H., Schwacke, L., Mcfee, W., and Kucklick, J., "The distribution and stratification of persistent organic pollutants and fatty acids in bottlenose dolphin (Tursiops truncatus) blubber," Sci. Total Environ., 463, 581-588 (2013).
  • Wilson, R. M., Kucklick, J. R., Balmer, B. C., Wells, R. S., Chanton, J. P., and Nowacek, D. P., "Spatial distribution of bottlenose dolphins (Tursiops truncatus) inferred from stable isotopes and priority organic pollutants," Sci. Total Environ., 425, 223-230 (2012).